Using C-13 solid-state NMR, encapsulation of C-60 within the nanotubular cavity was confirmed by downfield chemical shifts and significantly shorter spin spin relaxation times compared to C-60 physically mixed with s-PMMA. Thermal analysis revealed a melting endotherm at 180 degrees DMXAA mw C for the C-60/s-PMMA electrospun fibers that is consistent with formation of the nanotubular supramolecular complex. Based on the syndiotactic content of the s-PMMA and,the gravimetrically determined fullerene content in the electrospun fibers, 82% of the nanocavity is filled with C-60, dropping to 55% filling with C-70.

The fibers were macroscopically aligned by electrospinning onto a split collector plate for analysis www.selleckchem.com/products/lcl161.html of molecular alignment. Wide-angle X-ray scattering azimuthal scans revealed high degrees of molecular-level alignment, with an order parameter of 0.70 for the C-60/s-PMMA nanopods. (C) 2014 Elsevier Ltd. All rights reserved.”
“Cancer relies upon frequent or abnormal cell division, but how the tumor microenvironment affects mitotic processes in vivo remains unclear, largely due to the technical challenges

of optical access, spatial resolution, and motion. We developed high-resolution in vivo microscopy methods to visualize mitosis in a murine xenograft model of human cancer. Using these methods, we determined whether the single-cell response to the antimitotic drug paclitaxel (Ptx) was the same in tumors as in cell culture, observed the impact of Ptx on

the tumor response as a whole, and evaluated the single-cell pharmacodynamics (PD) of Ptx (by in vivo PD microscopy). Mitotic initiation was generally less frequent in tumors than in cell culture, but subsequently it proceeded normally. Ptx treatment caused spindle assembly defects and mitotic arrest, followed by slippage from mitotic arrest, multinucleation, and apoptosis. Compared with cell culture, the peak mitotic index in tumors exposed to Ptx was lower and the tumor cells survived longer after mitotic arrest, becoming multinucleated rather than dying directly from mitotic arrest. Thus, the tumor microenvironment was much less proapoptotic than cell MG-132 inhibitor culture. The morphologies associated with mitotic arrest were dose and time dependent, thereby providing a semiquantitative, single-cell measure of PD. Although many tumor cells did not progress through Ptx-induced mitotic arrest, tumor significantly regressed in the model. Our findings show that in vivo microscopy offers a useful tool to visualize mitosis during tumor progression, drug responses, and cell fate at the single-cell level. Cancer Res; 71(13); 4608-16. (C)2011 AACR.”
“Monascus fungi are commonly used for a variety of food products in Asia, and are also known to produce some biologically active compounds.