Circular RNA circ_0057558 Controls Prostate Cancer Cell Proliferation Through Regulating miR-206/USP33/c-Myc Axis
We formerly reported the improved expression of circ_0057558 in cancer of the prostate tissues and cell lines. Here, we aimed to look for the biological purpose of circ_0057558 in cancer of the prostate. In the present study, circ_0057558 knockdown in cancer of the prostate cells considerably repressed cell proliferation and colony formation, but promoted cell arrest that has been enhanced the sensitivity to docetaxel. Bioinformatics analysis conjecture and RNA-pull lower assay identified miR-206 because the potential binding miRNA of circ_0057558. An adverse correlation was observed between your expression of miR-206 and circ_0057558 in cancer of the prostate tissues. miR-206 mimics saved the part of circ_0057558 overexpression on cancer of the prostate cells. Further, the bioinformatics analysis and luciferase assay recommended that miR-206 may target ubiquitin-specific peptidase 33 (USP33). USP33 mRNA expression has negative correlation with miR-206 expression and positive correlation with circ_0057558 expression in cancer of the prostate tissues. USP33 overexpression partly blocked the results of miR-206 mimics on prostate cell proliferation. USP33 could bind and deubiquitinate c-Myc. Elevated c-Myc protein by circ_0057558 overexpression was partly reversed by miR-206 mimics. The proliferation inhibition activity of MYC inhibitor 361 (MYCi361) was more prominent in primary cancer of the prostate cells and patient-derived xenograft (PDX) model with greater degree of circ_0057558. With each other, circ_0057558 gives an impetus to cell proliferation and cell cycle control in cancer of the prostate cell lines by sponging miR-206 and positively controlling the transcription from the miR-206 target gene USP33.