After abrasion to the barge wall, platelets attach to the subendothelial cast and accumulated with one addition to anatomy a primary hemostatic plug. Phosphatidyl serine acknowledgment on the apparent of activated platelets provides a thrombogenic apparent for the activation of the agglomeration protease cascade. PS-exposed membranes enhance the catalytic ability of membrane-anchored tissue factor, the primary architect of the agglomeration avalanche that leads to the about-face of prothrombin to thrombin and fibrinogen to fibrin. Whether circulating platelets intrinsically acquire TF protein, however, is a debated question? Studies accept approved that monocyte-derived microparticles bind to activated platelets and activated platelets themselves absolution TF-positive microparticles.
Platelets from advantageous animal capacity accommodate TF pre-mRNA and action it to the complete archetype in acknowledgment to cellular activation. Activated platelets aswell TF protein, procoagulant activity, and advance claret array formation. The intracellular signaling alleyway that controls TF pre-mRNA splicing involves Cdc2-like kinase 1, an agitator not ahead accepted to be present or accomplish in platelets. Inhibition of Clk1 signaling in activated platelets blocks splicing agency 2/alternative splicing agency phosphorylation, TF pre-mRNA splicing, and de novo accession of bioactive TF protein.
Dr. Martin Buchwalter