Sclerostin mRNA in the liver was overex-pressed as compared with control samples (2.7±0.3 fold vs healthy liver). Sclerostin was detected by immunohistochemistry in 7 of the 11 liver samples but not in controls, and mainly located in the bile ducts. Sclerostin was directly Venetoclax datasheet associated with the severity of cholangitis (p=0.02)
and indirectly with the degree of lobular inflammation (p=0.03), but not with the degree of fibrosis neither with the Ludwig’s histologic stage. Sclerostin mRNA expression was higher in samples positive by immunohistochemistry (2.9 ±0.4vs 2.5 ± 0.3, p: n.s.), and particularly in those with lobular granuloma (3.6±0.6 vs 2.4±0.2, p=0.02). Conclusion: The increased expression of sclerostin in the liver and the association with histologic cholangitis may explain the high serum levels of this protein in patients with primary biliary cirrhosis, thus suggesting that sclerostin influences the decreased bone formation in this cholestatic disease. Disclosures: Albert Pares – Consulting: Lumena Pharmaceuticals The following people have nothing to disclose: Silvia Ruiz-Gaspa, Laia Gifre, Nuria Guañabens, Rosa Miquel, Pilar Peris, Ana Monegal [Background and
Aim] Drug-induced cholestasis is a rare, but sometimes, fatal disease. Underlying mechanisms remain unknown. Organic anion transporting polypeptides OATP1B1 (gene: SLCO1B1) and OATP1B3 (gene: SLCO1B3) were identified as responsible U0126 order learn more for Rotor syndrome. We have recently demonstrated that six Japanese
patients with Rotor syndrome were homozygous for the insertion of LINE-1 (L1) retrotransposon in intron 5 of SLCO1B3 and for c.1738C>T (p.R580X) nonsense mutation in SLCO1B1. Intronic L1 insertion in SLCO1B3 causes skipping of exon 5 or exons 5-7 in SLCO1B3 mRNA, yields immature stop codon, and results in the generation of truncated OATP1B3 protein. OATP1B1 and 1B3 are involved in hepatic uptake of bilirubin glucuronides, bile acids, and steroidal and thyroid hormones, as well as various drugs such as statins and anticancer drugs. Therefore, their genetic abnormalities may cause acquired cholestasis. In this study we analyzed L1 insertion in SLCO1B3 and c.1738C>T mutation in SLCO1B1 in Japanese patients with drug-induced cholestasis. [Patients and Methods] A total of 44 Japanese patients with drug-induced cholestasis were enrolled after written informed consent was obtained. Inclusion criteria were (1) alkaline phos-phatase (AP) greater than 2 times the upper limit of normal (ULN), (2) R ≤ 2, when R was defined as alanine aminotransferase (ALT)/ULN divided by AP/ULN, and (3) absence of other hepatobiliary diseases. A single-tube, three-primer PCR assay was established to detect L1 insertion in SLCO1B3. The c.1738C>T mutation in SLCO1B1 was genotyped by direct sequencing. Allele frequency was compared with Japanese controls (n=554). [Results] Median (min-max) age of the cases was 65 (21-80) years and males were dominant (64%).